Cells recall the way they were

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Illustration by Judy Blomquist

By Jessica Lau

Harvard Gazette

When cells grow up, they remember their childhoods.

A new study has found that adult cells keep a record of which genes were activated during their early development. Even more surprisingly, the memory is retrievable: Under certain lab conditions, cells can play the story of their development in reverse, switching on genes that were active before. The study, by researchers at the Dana-Farber Cancer Institute (DFCI), Brigham and Women’s Hospital, Harvard Medical School, and the Harvard Stem Cell Institute, was published in Molecular Cell.

Ramesh Shivdasani

“We discovered that adult cells maintain a catalog of all of the genes used early in development — a record when organs and tissues are formed within the embryo,” said senior author Ramesh Shivdasani, professor of medicine at Harvard Medical School and DFCI, and faculty member of the Harvard Stem Cell Institute.

“Beyond the sheer existence of this archive, we were surprised to find that it doesn’t remain permanently locked away but can be accessed by cells under certain conditions,” he said. “This discovery has potentially profound implications for how we think about cells’ capabilities, and for the future treatment of degenerative and other diseases.”

Playing the gene raffle

The many different cell types in the body all share the same DNA. Their differences come from how their genes are controlled. One control element is the enhancer, a region of DNA that turns genes on and off.

“The diversity of cell types comes from playing the same gene raffle in increasingly complicated ways,” said Shivdasani. “Evolution has given each gene multiple control elements.

“During development, cells continually make choices,” he added. “They go from an undifferentiated mass all the way to specific tissues and organs, using enhancers to make that happen.”

The enhancers used during development are different from the ones used in adult tissues. In this study, the researchers asked whether adult cells remember their developmental enhancers.

A complete and accessible memory

The researchers tracked mouse intestinal cells as they developed from the embryonic stage to adult tissue, seeing which enhancers were used when. They also looked at adult cell DNA, focusing on regions with a specific pattern of chemical modification. The two data sets matched: The pattern of chemical modification in the adult cells was a record of developmental enhancers.

“The idea that developmental enhancers are preserved in this form is not completely new,” said Shivdasani. “What we found was that the archive of enhancer activity is remarkably complete.”

The archive is not just comprehensive, it is also accessible. When researchers removed a certain protein from the cells, the developmental enhancers were reactivated accurately in reverse order. Underscoring the importance of curiosity-led research, the researchers did not set out to discover how cells “remember” development.

“We wanted to understand the basic biology of the protein complex PRC2, which silences genes when a cell’s fate is decided, because genes that make it are mutated in certain types of cancer,” said Unmesh Jadhav, an instructor in medicine at Harvard Medical School and DFCI. “We had no expectation that our work would lead to this find.”

“Discovering that this memory is completely recoverable was unexpected and profound,” added Shivdasani.

Using cell memory

Why do adult cells preserve these molecular memories? One possibility is that the memories are simply relics of cell development. Alternatively, cells may need to summon these memories in order to make new tissue and repair injuries.

“If the body needs to regenerate tissue that is damaged, it may be necessary for cells within that tissue to replay what happened in the embryo,” Shivdasani said.

This possibility holds promise for regenerative medicine. One day, patients’ cells may be coaxed into an earlier stage of development and then allowed to mature into adult tissues that can be used to replace diseased or failing organs. The study’s findings also hold promise for cancer research. Scientists have long thought that cancer cells are able to leave the original tumor and metastasize in part by switching on genes that were active during fetal development. Investigating the record of developmental enhancers may suggest new targets for therapies aimed at halting or preventing metastasis.

(Reprinted with permission from the Harvard Gazette.)

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